SOLiD™ SAGE™ Analysis Software

Description

• SOLiD™ SAGE™ Analysis Software is a tool for taking the raw sequence data files from SOLiD™ SAGE™ reads and matching them to known sequences in your reference database of choice. It is designed for use with the SOLiD™ SAGE™ kit, which generates libraries of 27-bp tags for all transcripts in a cell (catalog no. S3100301).Using the simple software interface, you create a project name and location, and then identify the directories containing:

  • SAGE™ tag sequences from a SOLiD™ instrument read (csfasta formatted)

  • A fasta-formatted database of reference sequences (e.g., from Ref Seq).

• The software then automatically matches the experimental data with the reference data, or compares two experimental data sets. Using the Corona Lite analysis engine, the software identifies tags, tabulates tag abundances, and displays associated gene descriptions. It presents the data in a tabular format that is easy to read and export to spreadsheet or other programs for further analysis.

Licensing

• This software is being licensed to you under the End User License Agreement which can be found in the SOLiD SAGE Software Guide.  Please read the license in its entirety and ensure that you understand the licensing conditions for use. Your use of this software indicates your acceptance of this licensing agreement. Click on this link to read the licensing agreement.

Support

• This tool is being released as "unsupported software" by Applied Biosystems.

Documentation

• Documentation for this pipeline can be found here

Software Download

• This software is distributed as source code.  The current version of the SOLiD™ SAGE Analysis Software can be downloaded here.

Sample Data Download

• Sample data for this software tool can be downloaded here.